Construct: shRNA TRCN0000056185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025202.2-781s1c1
DNA Barcode:
CCAGAAACTCAAGGCCAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFHD1 (80303)
Gene Description:
EF-hand domain family member D1
Transcript:
RefSeq NM_025202.2 (NON-CURRENT)
Match location:
Position 781 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAACTCAAGGCCAACTT
Hairpin Sequence:
5'-CCGG-CCAGAAACTCAAGGCCAACTT-CTCGAG-AAGTTGGCCTTGAGTTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAACTCAAGGCCAACTTCTCGAGAAGTTGGCCTTGAGTTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAACTCAAGGCCAACTTCTCGAGAAGTTGGCCTTGAGTTTCTGG-3'

Other clones with same target sequence:

(none)