Construct: shRNA TRCN0000056204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002670.1-1756s1c1
DNA Barcode:
CCTAGATTTAATAGATGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLS1 (5357)
Gene Description:
plastin 1
Transcript:
RefSeq NM_002670.1 (NON-CURRENT)
Match location:
Position 1756 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGATTTAATAGATGCCAT
Hairpin Sequence:
5'-CCGG-CCTAGATTTAATAGATGCCAT-CTCGAG-ATGGCATCTATTAAATCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGATTTAATAGATGCCATCTCGAGATGGCATCTATTAAATCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGATTTAATAGATGCCATCTCGAGATGGCATCTATTAAATCTAGG-3'

Other clones with same target sequence:

  1. TRCN0000363755