Construct: shRNA TRCN0000056271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021105.1-1094s1c1
DNA Barcode:
CAGTTCCCTTTAGACCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLSCR1 (5359)
Gene Description:
phospholipid scramblase 1
Transcript:
RefSeq NM_021105.1 (NON-CURRENT)
Match location:
Position 1094 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTCCCTTTAGACCTTGAT
Hairpin Sequence:
5'-CCGG-CAGTTCCCTTTAGACCTTGAT-CTCGAG-ATCAAGGTCTAAAGGGAACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTCCCTTTAGACCTTGATCTCGAGATCAAGGTCTAAAGGGAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTCCCTTTAGACCTTGATCTCGAGATCAAGGTCTAAAGGGAACTG-3'

Other clones with same target sequence:

  1. TRCN0000299123