Construct: shRNA TRCN0000056291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014386.1-1592s1c1
DNA Barcode:
CGAGAAATTCAGACTGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKD2L2 (27039)
Gene Description:
polycystin 2 like 2, transient receptor potential cation channel
Transcript:
RefSeq NM_014386.1 (NON-CURRENT)
Match location:
Position 1592 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAAATTCAGACTGAAGAA
Hairpin Sequence:
5'-CCGG-CGAGAAATTCAGACTGAAGAA-CTCGAG-TTCTTCAGTCTGAATTTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAAATTCAGACTGAAGAACTCGAGTTCTTCAGTCTGAATTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAAATTCAGACTGAAGAACTCGAGTTCTTCAGTCTGAATTTCTCG-3'

Other clones with same target sequence:

(none)