Construct: shRNA TRCN0000056294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198994.1-1729s1c1
DNA Barcode:
GCCGCAAGAAGAGAAGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TGM6 (343641)
Gene Description:
transglutaminase 6
Transcript:
RefSeq NM_198994.1 (NON-CURRENT)
Match location:
Position 1729 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCAAGAAGAGAAGAGAAT
Hairpin Sequence:
5'-CCGG-GCCGCAAGAAGAGAAGAGAAT-CTCGAG-ATTCTCTTCTCTTCTTGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCAAGAAGAGAAGAGAATCTCGAGATTCTCTTCTCTTCTTGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCAAGAAGAGAAGAGAATCTCGAGATTCTCTTCTCTTCTTGCGGC-3'

Other clones with same target sequence:

(none)