Construct: shRNA TRCN0000056383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031935.1-12552s1c1
DNA Barcode:
CGCCTGACATTACATGGCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMCN1 (83872)
Gene Description:
hemicentin 1
Transcript:
RefSeq NM_031935.1 (NON-CURRENT)
Match location:
Position 12552 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTGACATTACATGGCATA
Hairpin Sequence:
5'-CCGG-CGCCTGACATTACATGGCATA-CTCGAG-TATGCCATGTAATGTCAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTGACATTACATGGCATACTCGAGTATGCCATGTAATGTCAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTGACATTACATGGCATACTCGAGTATGCCATGTAATGTCAGGCG-3'

Other clones with same target sequence:

(none)