Construct: shRNA TRCN0000056486

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033026.2-1600s1c1
DNA Barcode:
CCCTTATGATAGCACCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCLO (27445)
Gene Description:
piccolo presynaptic cytomatrix protein
Transcript:
RefSeq NM_033026.2 (NON-CURRENT)
Match location:
Position 1600 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056486)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATGATAGCACCTGTTT
Hairpin Sequence:
5'-CCGG-CCCTTATGATAGCACCTGTTT-CTCGAG-AAACAGGTGCTATCATAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATGATAGCACCTGTTTCTCGAGAAACAGGTGCTATCATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATGATAGCACCTGTTTCTCGAGAAACAGGTGCTATCATAAGGG-3'

Other clones with same target sequence:

(none)