Construct: shRNA TRCN0000056532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371257.1-2020s1c1
DNA Barcode:
CTCAACATATTTGCAGTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFCAB7 (84455)
Gene Description:
EF-hand calcium binding domain 7
Transcript:
RefSeq XM_371257.1 (NON-CURRENT)
Match location:
Position 2020 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACATATTTGCAGTAGAA
Hairpin Sequence:
5'-CCGG-CTCAACATATTTGCAGTAGAA-CTCGAG-TTCTACTGCAAATATGTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACATATTTGCAGTAGAACTCGAGTTCTACTGCAAATATGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACATATTTGCAGTAGAACTCGAGTTCTACTGCAAATATGTTGAG-3'

Other clones with same target sequence:

(none)