Construct: shRNA TRCN0000056638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016082.3-938s1c1
DNA Barcode:
CCAATCTCAGTCGTGGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK5RAP1 (51654)
Gene Description:
CDK5 regulatory subunit associated protein 1
Transcript:
RefSeq NM_016082.3 (NON-CURRENT)
Match location:
Position 938 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCTCAGTCGTGGCTTTA
Hairpin Sequence:
5'-CCGG-CCAATCTCAGTCGTGGCTTTA-CTCGAG-TAAAGCCACGACTGAGATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCTCAGTCGTGGCTTTACTCGAGTAAAGCCACGACTGAGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCTCAGTCGTGGCTTTACTCGAGTAAAGCCACGACTGAGATTGG-3'

Other clones with same target sequence:

(none)