Construct: shRNA TRCN0000056672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080657.3-147s1c1
DNA Barcode:
GCTAGCTACCAAGAGGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSAD2 (91543)
Gene Description:
radical S-adenosyl methionine domain containing 2
Transcript:
RefSeq NM_080657.3 (NON-CURRENT)
Match location:
Position 147 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGCTACCAAGAGGAGAAA
Hairpin Sequence:
5'-CCGG-GCTAGCTACCAAGAGGAGAAA-CTCGAG-TTTCTCCTCTTGGTAGCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGCTACCAAGAGGAGAAACTCGAGTTTCTCCTCTTGGTAGCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGCTACCAAGAGGAGAAACTCGAGTTTCTCCTCTTGGTAGCTAGC-3'

Other clones with same target sequence:

(none)