Construct: shRNA TRCN0000056673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173353.2-1904s1c1
DNA Barcode:
CCCTCTTATGAGTCTCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPH2 (121278)
Gene Description:
tryptophan hydroxylase 2
Transcript:
RefSeq NM_173353.2 (NON-CURRENT)
Match location:
Position 1904 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTTATGAGTCTCATTTA
Hairpin Sequence:
5'-CCGG-CCCTCTTATGAGTCTCATTTA-CTCGAG-TAAATGAGACTCATAAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTTATGAGTCTCATTTACTCGAGTAAATGAGACTCATAAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTTATGAGTCTCATTTACTCGAGTAAATGAGACTCATAAGAGGG-3'

Other clones with same target sequence:

(none)