Construct: shRNA TRCN0000056679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173640.1-402s1c1
DNA Barcode:
GACATGAACAAGTGCATCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSPO1 (284654)
Gene Description:
R-spondin 1
Transcript:
RefSeq NM_173640.1 (NON-CURRENT)
Match location:
Position 402 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATGAACAAGTGCATCAAA
Hairpin Sequence:
5'-CCGG-GACATGAACAAGTGCATCAAA-CTCGAG-TTTGATGCACTTGTTCATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATGAACAAGTGCATCAAACTCGAGTTTGATGCACTTGTTCATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATGAACAAGTGCATCAAACTCGAGTTTGATGCACTTGTTCATGTC-3'

Other clones with same target sequence:

(none)