Construct: shRNA TRCN0000056680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173640.1-320s1c1
DNA Barcode:
CCTGCTGGAGAGGAACGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSPO1 (284654)
Gene Description:
R-spondin 1
Transcript:
RefSeq NM_173640.1 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTGGAGAGGAACGACAT
Hairpin Sequence:
5'-CCGG-CCTGCTGGAGAGGAACGACAT-CTCGAG-ATGTCGTTCCTCTCCAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTGGAGAGGAACGACATCTCGAGATGTCGTTCCTCTCCAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTGGAGAGGAACGACATCTCGAGATGTCGTTCCTCTCCAGCAGG-3'

Other clones with same target sequence:

(none)