Construct: shRNA TRCN0000056778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080745.2-674s1c1
DNA Barcode:
CCACTAACTCTGGACCCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM69 (140691)
Gene Description:
tripartite motif containing 69
Transcript:
RefSeq NM_080745.2 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTAACTCTGGACCCTAAA
Hairpin Sequence:
5'-CCGG-CCACTAACTCTGGACCCTAAA-CTCGAG-TTTAGGGTCCAGAGTTAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTAACTCTGGACCCTAAACTCGAGTTTAGGGTCCAGAGTTAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTAACTCTGGACCCTAAACTCGAGTTTAGGGTCCAGAGTTAGTGG-3'

Other clones with same target sequence:

(none)