Construct: shRNA TRCN0000056784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006864.1-622s1c1
DNA Barcode:
GTGGAGGTTCACATGCTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LILRB3 (11025)
Gene Description:
leukocyte immunoglobulin like receptor B3
Transcript:
RefSeq NM_006864.1 (NON-CURRENT)
Match location:
Position 622 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAGGTTCACATGCTATTA
Hairpin Sequence:
5'-CCGG-GTGGAGGTTCACATGCTATTA-CTCGAG-TAATAGCATGTGAACCTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAGGTTCACATGCTATTACTCGAGTAATAGCATGTGAACCTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAGGTTCACATGCTATTACTCGAGTAATAGCATGTGAACCTCCAC-3'

Other clones with same target sequence:

(none)