Construct: shRNA TRCN0000056828
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_181784.1-548s1c1
- DNA Barcode:
- GCAATCGAAGACCTTATAGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPRED2 (200734)
- Gene Description:
- sprouty related EVH1 domain containing 2
- Transcript:
- RefSeq NM_181784.1 (NON-CURRENT)
- Match location:
- Position 548 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000056828)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAATCGAAGACCTTATAGAA
- Hairpin Sequence:
-
5'-CCGG-GCAATCGAAGACCTTATAGAA-CTCGAG-TTCTATAAGGTCTTCGATTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAATCGAAGACCTTATAGAACTCGAGTTCTATAAGGTCTTCGATTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAATCGAAGACCTTATAGAACTCGAGTTCTATAAGGTCTTCGATTGC-3'
Other clones with same target sequence:
(none)
