Construct: shRNA TRCN0000056883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005658.3-3414s1c1
DNA Barcode:
GCAGTCTCAATGGGTCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAF1 (7185)
Gene Description:
TNF receptor associated factor 1
Transcript:
RefSeq NM_005658.3 (NON-CURRENT)
Match location:
Position 3414 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCTCAATGGGTCAGAAA
Hairpin Sequence:
5'-CCGG-GCAGTCTCAATGGGTCAGAAA-CTCGAG-TTTCTGACCCATTGAGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCTCAATGGGTCAGAAACTCGAGTTTCTGACCCATTGAGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCTCAATGGGTCAGAAACTCGAGTTTCTGACCCATTGAGACTGC-3'

Other clones with same target sequence:

(none)