Construct: shRNA TRCN0000056929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014513.1-659s1c1
DNA Barcode:
GCTTGTTTCTGTCACAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR2DS5 (3810)
Gene Description:
killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 5
Transcript:
RefSeq NM_014513.1 (NON-CURRENT)
Match location:
Position 659 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTTTCTGTCACAGGAAA
Hairpin Sequence:
5'-CCGG-GCTTGTTTCTGTCACAGGAAA-CTCGAG-TTTCCTGTGACAGAAACAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTTTCTGTCACAGGAAACTCGAGTTTCCTGTGACAGAAACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTTTCTGTCACAGGAAACTCGAGTTTCCTGTGACAGAAACAAGC-3'

Other clones with same target sequence:

(none)