Construct: shRNA TRCN0000056957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003268.3-2188s1c1
DNA Barcode:
CCCTTCCACCAGGAGTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLR5 (7100)
Gene Description:
toll like receptor 5
Transcript:
RefSeq NM_003268.3 (NON-CURRENT)
Match location:
Position 2188 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCCACCAGGAGTATTTA
Hairpin Sequence:
5'-CCGG-CCCTTCCACCAGGAGTATTTA-CTCGAG-TAAATACTCCTGGTGGAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCCACCAGGAGTATTTACTCGAGTAAATACTCCTGGTGGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCCACCAGGAGTATTTACTCGAGTAAATACTCCTGGTGGAAGGG-3'

Other clones with same target sequence:

(none)