Construct: shRNA TRCN0000056989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014512.1-598s1c1
DNA Barcode:
CTACAGATGCTTCGGCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR2DS1 (3806)
Gene Description:
killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 1
Transcript:
RefSeq NM_014512.1 (CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAGATGCTTCGGCTCTTT
Hairpin Sequence:
5'-CCGG-CTACAGATGCTTCGGCTCTTT-CTCGAG-AAAGAGCCGAAGCATCTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAGATGCTTCGGCTCTTTCTCGAGAAAGAGCCGAAGCATCTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAGATGCTTCGGCTCTTTCTCGAGAAAGAGCCGAAGCATCTGTAG-3'

Other clones with same target sequence:

(none)