Construct: shRNA TRCN0000057031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012312.1-157s1c1
DNA Barcode:
CCTGCAATGTTGGTCAGATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR2DS2 (3807)
Gene Description:
killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 2
Transcript:
RefSeq NM_012312.1 (NON-CURRENT)
Match location:
Position 157 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAATGTTGGTCAGATGT
Hairpin Sequence:
5'-CCGG-CCTGCAATGTTGGTCAGATGT-CTCGAG-ACATCTGACCAACATTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAATGTTGGTCAGATGTCTCGAGACATCTGACCAACATTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAATGTTGGTCAGATGTCTCGAGACATCTGACCAACATTGCAGG-3'

Other clones with same target sequence:

(none)