Construct: shRNA TRCN0000057136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000186.1-290s1c1
DNA Barcode:
GCTCTTAATCCATTAAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFH (3075)
Gene Description:
complement factor H
Transcript:
RefSeq NM_000186.1 (NON-CURRENT)
Match location:
Position 290 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTAATCCATTAAGGAAA
Hairpin Sequence:
5'-CCGG-GCTCTTAATCCATTAAGGAAA-CTCGAG-TTTCCTTAATGGATTAAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTAATCCATTAAGGAAACTCGAGTTTCCTTAATGGATTAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTAATCCATTAAGGAAACTCGAGTTTCCTTAATGGATTAAGAGC-3'

Other clones with same target sequence:

(none)