Construct: shRNA TRCN0000057177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000587.2-990s1c1
DNA Barcode:
CCGAAGATTAATCGACCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C7 (730)
Gene Description:
complement C7
Transcript:
RefSeq NM_000587.2 (NON-CURRENT)
Match location:
Position 990 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAGATTAATCGACCAGTA
Hairpin Sequence:
5'-CCGG-CCGAAGATTAATCGACCAGTA-CTCGAG-TACTGGTCGATTAATCTTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAGATTAATCGACCAGTACTCGAGTACTGGTCGATTAATCTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAGATTAATCGACCAGTACTCGAGTACTGGTCGATTAATCTTCGG-3'

Other clones with same target sequence:

(none)