Construct: shRNA TRCN0000057230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000734.2-463s1c1
DNA Barcode:
GCAGAAAGATAAGATGGCGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD247 (919)
Gene Description:
CD247 molecule
Transcript:
RefSeq NM_000734.2 (NON-CURRENT)
Match location:
Position 463 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAAGATAAGATGGCGGA
Hairpin Sequence:
5'-CCGG-GCAGAAAGATAAGATGGCGGA-CTCGAG-TCCGCCATCTTATCTTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAAGATAAGATGGCGGACTCGAGTCCGCCATCTTATCTTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAAGATAAGATGGCGGACTCGAGTCCGCCATCTTATCTTTCTGC-3'

Other clones with same target sequence:

(none)