Construct: shRNA TRCN0000057257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004048.2-217s1c1
DNA Barcode:
TCCGACATTGAAGTTGACTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B2M (567)
Gene Description:
beta-2-microglobulin
Transcript:
RefSeq NM_004048.2 (NON-CURRENT)
Match location:
Position 217 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGACATTGAAGTTGACTTA
Hairpin Sequence:
5'-CCGG-TCCGACATTGAAGTTGACTTA-CTCGAG-TAAGTCAACTTCAATGTCGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGACATTGAAGTTGACTTACTCGAGTAAGTCAACTTCAATGTCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGACATTGAAGTTGACTTACTCGAGTAAGTCAACTTCAATGTCGGA-3'

Other clones with same target sequence:

  1. TRCN0000299088