Construct: shRNA TRCN0000057359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006438.2-691s1c1
DNA Barcode:
CGGGTGTTCATTGGCGTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COLEC10 (10584)
Gene Description:
collectin subfamily member 10
Transcript:
RefSeq NM_006438.2 (NON-CURRENT)
Match location:
Position 691 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTGTTCATTGGCGTGAAT
Hairpin Sequence:
5'-CCGG-CGGGTGTTCATTGGCGTGAAT-CTCGAG-ATTCACGCCAATGAACACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTGTTCATTGGCGTGAATCTCGAGATTCACGCCAATGAACACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTGTTCATTGGCGTGAATCTCGAGATTCACGCCAATGAACACCCG-3'

Other clones with same target sequence:

(none)