Construct: shRNA TRCN0000057731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002203.2-1427s1c1
DNA Barcode:
CCGGCCAGATAGTGCTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITGA2 (3673)
Gene Description:
integrin subunit alpha 2
Transcript:
RefSeq NM_002203.2 (NON-CURRENT)
Match location:
Position 1427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGCCAGATAGTGCTATATA
Hairpin Sequence:
5'-CCGG-CCGGCCAGATAGTGCTATATA-CTCGAG-TATATAGCACTATCTGGCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGCCAGATAGTGCTATATACTCGAGTATATAGCACTATCTGGCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGCCAGATAGTGCTATATACTCGAGTATATAGCACTATCTGGCCGG-3'

Other clones with same target sequence:

  1. TRCN0000289151