Construct: shRNA TRCN0000057783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002208.3-2589s1c1
DNA Barcode:
GCCTGCAAGAATAAGCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITGAE (3682)
Gene Description:
integrin subunit alpha E
Transcript:
RefSeq NM_002208.3 (NON-CURRENT)
Match location:
Position 2589 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000057783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCAAGAATAAGCTGTTT
Hairpin Sequence:
5'-CCGG-GCCTGCAAGAATAAGCTGTTT-CTCGAG-AAACAGCTTATTCTTGCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCAAGAATAAGCTGTTTCTCGAGAAACAGCTTATTCTTGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCAAGAATAAGCTGTTTCTCGAGAAACAGCTTATTCTTGCAGGC-3'

Other clones with same target sequence:

(none)