Construct: shRNA TRCN0000058070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005789.2-485s1c1
DNA Barcode:
CGAAGGTTGGATGAGTGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSME3 (10197)
Gene Description:
proteasome activator subunit 3
Transcript:
RefSeq NM_005789.2 (NON-CURRENT)
Match location:
Position 485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGGTTGGATGAGTGTGAA
Hairpin Sequence:
5'-CCGG-CGAAGGTTGGATGAGTGTGAA-CTCGAG-TTCACACTCATCCAACCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGGTTGGATGAGTGTGAACTCGAGTTCACACTCATCCAACCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGGTTGGATGAGTGTGAACTCGAGTTCACACTCATCCAACCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000307160