Construct: shRNA TRCN0000058086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003650.2-612s1c1
DNA Barcode:
CCAAACCAACCACACCTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CST7 (8530)
Gene Description:
cystatin F
Transcript:
RefSeq NM_003650.2 (NON-CURRENT)
Match location:
Position 612 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACCAACCACACCTTGAA
Hairpin Sequence:
5'-CCGG-CCAAACCAACCACACCTTGAA-CTCGAG-TTCAAGGTGTGGTTGGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACCAACCACACCTTGAACTCGAGTTCAAGGTGTGGTTGGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACCAACCACACCTTGAACTCGAGTTCAAGGTGTGGTTGGTTTGG-3'

Other clones with same target sequence:

(none)