Construct: shRNA TRCN0000058104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002807.2-2963s1c1
DNA Barcode:
CCCAGAACCATTTGAGTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMD1 (5707)
Gene Description:
proteasome 26S subunit, non-ATPase 1
Transcript:
RefSeq NM_002807.2 (NON-CURRENT)
Match location:
Position 2963 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAACCATTTGAGTATAT
Hairpin Sequence:
5'-CCGG-CCCAGAACCATTTGAGTATAT-CTCGAG-ATATACTCAAATGGTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAACCATTTGAGTATATCTCGAGATATACTCAAATGGTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAACCATTTGAGTATATCTCGAGATATACTCAAATGGTTCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000290016