Construct: shRNA TRCN0000058109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002817.2-225s1c1
DNA Barcode:
CCCAAGGAGATGGTCTCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMD13 (5719)
Gene Description:
proteasome 26S subunit, non-ATPase 13
Transcript:
RefSeq NM_002817.2 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGGAGATGGTCTCATTA
Hairpin Sequence:
5'-CCGG-CCCAAGGAGATGGTCTCATTA-CTCGAG-TAATGAGACCATCTCCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGGAGATGGTCTCATTACTCGAGTAATGAGACCATCTCCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGGAGATGGTCTCATTACTCGAGTAATGAGACCATCTCCTTGGG-3'

Other clones with same target sequence:

(none)