Construct: shRNA TRCN0000058137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022367.2-1215s1c1
DNA Barcode:
GCCTTCTCTCTCTTGGACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA4A (64218)
Gene Description:
semaphorin 4A
Transcript:
RefSeq NM_022367.2 (NON-CURRENT)
Match location:
Position 1215 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTCTCTCTTGGACATT
Hairpin Sequence:
5'-CCGG-GCCTTCTCTCTCTTGGACATT-CTCGAG-AATGTCCAAGAGAGAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTCTCTCTTGGACATTCTCGAGAATGTCCAAGAGAGAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTCTCTCTTGGACATTCTCGAGAATGTCCAAGAGAGAGAAGGC-3'

Other clones with same target sequence:

(none)