Construct: shRNA TRCN0000058151

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153012.1-316s1c1
DNA Barcode:
CAGACAGAAGAAAGCCAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFSF12 (8742)
Gene Description:
TNF superfamily member 12
Transcript:
RefSeq NM_153012.1 (NON-CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058151)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACAGAAGAAAGCCAGGAT
Hairpin Sequence:
5'-CCGG-CAGACAGAAGAAAGCCAGGAT-CTCGAG-ATCCTGGCTTTCTTCTGTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACAGAAGAAAGCCAGGATCTCGAGATCCTGGCTTTCTTCTGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACAGAAGAAAGCCAGGATCTCGAGATCCTGGCTTTCTTCTGTCTG-3'

Other clones with same target sequence:

(none)