Construct: shRNA TRCN0000058158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001559.2-951s1c1
DNA Barcode:
CCTGTATCAATAGTGATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL12RB2 (3595)
Gene Description:
interleukin 12 receptor subunit beta 2
Transcript:
RefSeq NM_001559.2 (CURRENT)
Match location:
Position 951 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTATCAATAGTGATGAAA
Hairpin Sequence:
5'-CCGG-CCTGTATCAATAGTGATGAAA-CTCGAG-TTTCATCACTATTGATACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTATCAATAGTGATGAAACTCGAGTTTCATCACTATTGATACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTATCAATAGTGATGAAACTCGAGTTTCATCACTATTGATACAGG-3'

Other clones with same target sequence:

(none)