Construct: shRNA TRCN0000058249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006140.3-1109s1c1
DNA Barcode:
CCTGGAGTGAAGCCATTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSF2RA (1438)
Gene Description:
colony stimulating factor 2 receptor alpha subunit
Transcript:
RefSeq NM_006140.3 (NON-CURRENT)
Match location:
Position 1109 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGTGAAGCCATTGAAT
Hairpin Sequence:
5'-CCGG-CCTGGAGTGAAGCCATTGAAT-CTCGAG-ATTCAATGGCTTCACTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGTGAAGCCATTGAATCTCGAGATTCAATGGCTTCACTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGTGAAGCCATTGAATCTCGAGATTCAATGGCTTCACTCCAGG-3'

Other clones with same target sequence:

(none)