Construct: shRNA TRCN0000058435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005535.1-1300s1c1
DNA Barcode:
GTGTTACTACATTACCATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL12RB1 (3594)
Gene Description:
interleukin 12 receptor subunit beta 1
Transcript:
RefSeq NM_005535.1 (NON-CURRENT)
Match location:
Position 1300 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTACTACATTACCATCTT
Hairpin Sequence:
5'-CCGG-GTGTTACTACATTACCATCTT-CTCGAG-AAGATGGTAATGTAGTAACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTACTACATTACCATCTTCTCGAGAAGATGGTAATGTAGTAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTACTACATTACCATCTTCTCGAGAAGATGGTAATGTAGTAACAC-3'

Other clones with same target sequence:

(none)