Construct: shRNA TRCN0000058490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001720.3-1342s1c1
DNA Barcode:
CCAAGGCTACTCGGCCTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMP8B (656)
Gene Description:
bone morphogenetic protein 8b
Transcript:
RefSeq NM_001720.3 (NON-CURRENT)
Match location:
Position 1342 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGCTACTCGGCCTATTA
Hairpin Sequence:
5'-CCGG-CCAAGGCTACTCGGCCTATTA-CTCGAG-TAATAGGCCGAGTAGCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGCTACTCGGCCTATTACTCGAGTAATAGGCCGAGTAGCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGCTACTCGGCCTATTACTCGAGTAATAGGCCGAGTAGCCTTGG-3'

Other clones with same target sequence:

(none)