Construct: shRNA TRCN0000058491
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001720.3-745s1c1
- DNA Barcode:
- CCATTGGAAGGAGTTCCGCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMP8B (656)
- Gene Description:
- bone morphogenetic protein 8b
- Transcript:
- RefSeq NM_001720.3 (NON-CURRENT)
- Match location:
- Position 745 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000058491)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATTGGAAGGAGTTCCGCTT
- Hairpin Sequence:
-
5'-CCGG-CCATTGGAAGGAGTTCCGCTT-CTCGAG-AAGCGGAACTCCTTCCAATGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATTGGAAGGAGTTCCGCTTCTCGAGAAGCGGAACTCCTTCCAATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATTGGAAGGAGTTCCGCTTCTCGAGAAGCGGAACTCCTTCCAATGG-3'
Other clones with same target sequence:
(none)
