Construct: shRNA TRCN0000058523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000640.2-1148s1c1
DNA Barcode:
GCTACGTTTCTGGCTACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL13RA2 (3598)
Gene Description:
interleukin 13 receptor subunit alpha 2
Transcript:
RefSeq NM_000640.2 (NON-CURRENT)
Match location:
Position 1148 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGTTTCTGGCTACCATT
Hairpin Sequence:
5'-CCGG-GCTACGTTTCTGGCTACCATT-CTCGAG-AATGGTAGCCAGAAACGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGTTTCTGGCTACCATTCTCGAGAATGGTAGCCAGAAACGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGTTTCTGGCTACCATTCTCGAGAATGGTAGCCAGAAACGTAGC-3'

Other clones with same target sequence:

(none)