Construct: shRNA TRCN0000058537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000515.3-575s1c1
DNA Barcode:
GTTCGACACAAACTCACACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GH1 (2688)
Gene Description:
growth hormone 1
Transcript:
RefSeq NM_000515.3 (NON-CURRENT)
Match location:
Position 575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGACACAAACTCACACAA
Hairpin Sequence:
5'-CCGG-GTTCGACACAAACTCACACAA-CTCGAG-TTGTGTGAGTTTGTGTCGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGACACAAACTCACACAACTCGAGTTGTGTGAGTTTGTGTCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGACACAAACTCACACAACTCGAGTTGTGTGAGTTTGTGTCGAAC-3'

Other clones with same target sequence:

(none)