Construct: shRNA TRCN0000058650

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017563.1-1052s1c1
DNA Barcode:
GCCGCAAGAAGCAACAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL17RD (54756)
Gene Description:
interleukin 17 receptor D
Transcript:
RefSeq NM_017563.1 (NON-CURRENT)
Match location:
Position 1052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058650)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCAAGAAGCAACAAGAAA
Hairpin Sequence:
5'-CCGG-GCCGCAAGAAGCAACAAGAAA-CTCGAG-TTTCTTGTTGCTTCTTGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCAAGAAGCAACAAGAAACTCGAGTTTCTTGTTGCTTCTTGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCAAGAAGCAACAAGAAACTCGAGTTTCTTGTTGCTTCTTGCGGC-3'

Other clones with same target sequence:

(none)