Construct: shRNA TRCN0000058693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000418.1-619s1c1
DNA Barcode:
CCCTGACAATTACCTGTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL4R (3566)
Gene Description:
interleukin 4 receptor
Transcript:
RefSeq NM_000418.1 (NON-CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGACAATTACCTGTATAA
Hairpin Sequence:
5'-CCGG-CCCTGACAATTACCTGTATAA-CTCGAG-TTATACAGGTAATTGTCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGACAATTACCTGTATAACTCGAGTTATACAGGTAATTGTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGACAATTACCTGTATAACTCGAGTTATACAGGTAATTGTCAGGG-3'

Other clones with same target sequence:

(none)