Construct: shRNA TRCN0000058772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002310.2-3335s1c1
DNA Barcode:
GCTCCATTAATTCCCGACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIFR (3977)
Gene Description:
LIF receptor subunit alpha
Transcript:
RefSeq NM_002310.2 (NON-CURRENT)
Match location:
Position 3335 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCATTAATTCCCGACAAT
Hairpin Sequence:
5'-CCGG-GCTCCATTAATTCCCGACAAT-CTCGAG-ATTGTCGGGAATTAATGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCATTAATTCCCGACAATCTCGAGATTGTCGGGAATTAATGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCATTAATTCCCGACAATCTCGAGATTGTCGGGAATTAATGGAGC-3'

Other clones with same target sequence:

(none)