Construct: shRNA TRCN0000058799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002303.2-1102s1c1
DNA Barcode:
CGTGTCTTTACCACACAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LEPR (3953)
Gene Description:
leptin receptor
Transcript:
RefSeq NM_002303.2 (NON-CURRENT)
Match location:
Position 1102 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTCTTTACCACACAAGAT
Hairpin Sequence:
5'-CCGG-CGTGTCTTTACCACACAAGAT-CTCGAG-ATCTTGTGTGGTAAAGACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTCTTTACCACACAAGATCTCGAGATCTTGTGTGGTAAAGACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTCTTTACCACACAAGATCTCGAGATCTTGTGTGGTAAAGACACG-3'

Other clones with same target sequence:

(none)