Construct: shRNA TRCN0000058991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170743.2-1158s1c1
DNA Barcode:
CTTGGGATTCTTCAGACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFNLR1 (163702)
Gene Description:
interferon lambda receptor 1
Transcript:
RefSeq NM_170743.2 (NON-CURRENT)
Match location:
Position 1158 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000058991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGGATTCTTCAGACAGAA
Hairpin Sequence:
5'-CCGG-CTTGGGATTCTTCAGACAGAA-CTCGAG-TTCTGTCTGAAGAATCCCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGGATTCTTCAGACAGAACTCGAGTTCTGTCTGAAGAATCCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGGATTCTTCAGACAGAACTCGAGTTCTGTCTGAAGAATCCCAAG-3'

Other clones with same target sequence:

(none)