Construct: shRNA TRCN0000059046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020740.1-688s1c1
DNA Barcode:
GCCATCAAAGTGGAGAGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKFY1 (51479)
Gene Description:
ankyrin repeat and FYVE domain containing 1
Transcript:
RefSeq NM_020740.1 (NON-CURRENT)
Match location:
Position 688 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCAAAGTGGAGAGAGAA
Hairpin Sequence:
5'-CCGG-GCCATCAAAGTGGAGAGAGAA-CTCGAG-TTCTCTCTCCACTTTGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCAAAGTGGAGAGAGAACTCGAGTTCTCTCTCCACTTTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCAAAGTGGAGAGAGAACTCGAGTTCTCTCTCCACTTTGATGGC-3'

Other clones with same target sequence:

(none)