Construct: shRNA TRCN0000059182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022148.2-524s1c1
DNA Barcode:
GTCACCATAGAAGGCTTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRLF2 (64109)
Gene Description:
cytokine receptor like factor 2
Transcript:
RefSeq NM_022148.2 (NON-CURRENT)
Match location:
Position 524 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACCATAGAAGGCTTGGAT
Hairpin Sequence:
5'-CCGG-GTCACCATAGAAGGCTTGGAT-CTCGAG-ATCCAAGCCTTCTATGGTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACCATAGAAGGCTTGGATCTCGAGATCCAAGCCTTCTATGGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACCATAGAAGGCTTGGATCTCGAGATCCAAGCCTTCTATGGTGAC-3'

Other clones with same target sequence:

(none)