Construct: shRNA TRCN0000059260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000877.2-1022s1c1
DNA Barcode:
GCCAAGAATACACATGGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL1R1 (3554)
Gene Description:
interleukin 1 receptor type 1
Transcript:
RefSeq NM_000877.2 (NON-CURRENT)
Match location:
Position 1022 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAATACACATGGTATA
Hairpin Sequence:
5'-CCGG-GCCAAGAATACACATGGTATA-CTCGAG-TATACCATGTGTATTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAATACACATGGTATACTCGAGTATACCATGTGTATTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAATACACATGGTATACTCGAGTATACCATGTGTATTCTTGGC-3'

Other clones with same target sequence:

(none)