Construct: shRNA TRCN0000059271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033226.1-767s1c1
DNA Barcode:
CCGGTGATTCTCATTCACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCC12 (94160)
Gene Description:
ATP binding cassette subfamily C member 12
Transcript:
RefSeq NM_033226.1 (NON-CURRENT)
Match location:
Position 767 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGTGATTCTCATTCACCAA
Hairpin Sequence:
5'-CCGG-CCGGTGATTCTCATTCACCAA-CTCGAG-TTGGTGAATGAGAATCACCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGTGATTCTCATTCACCAACTCGAGTTGGTGAATGAGAATCACCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGTGATTCTCATTCACCAACTCGAGTTGGTGAATGAGAATCACCGG-3'

Other clones with same target sequence:

(none)